http://glutamate.med.kyoto-u.ac.jp/e/index.php?action=history&feed=atom&title=Tomohisa_HosokawaTomohisa Hosokawa - Revision history2026-04-10T16:28:51ZRevision history for this page on the wikiMediaWiki 1.43.0http://glutamate.med.kyoto-u.ac.jp/e/index.php?title=Tomohisa_Hosokawa&diff=415&oldid=prevYasunori Hayashi: Created page with "== Bibliography == *2005 Graduated from Department of Biological sciences, Tokyo Metropolitan University *2010 Ph.D. Graduate School of Sciences, Tokyo Metropolitan University *2010-2017 Post-doc, Lab. for Memory Mechanisms, Brain Science Institute, RIKEN. *2017-2021 Post-doc, Lab. for systems neuropharmacology, Department of Pharmacology, Graduate school of medicine, Kyoto University. *2021-2023 Lecturer, Lab. for Cell regulation, Graduate school of Sciences, Nagoya Uni..."2025-03-24T00:50:49Z<p>Created page with "== Bibliography == *2005 Graduated from Department of Biological sciences, Tokyo Metropolitan University *2010 Ph.D. Graduate School of Sciences, Tokyo Metropolitan University *2010-2017 Post-doc, Lab. for Memory Mechanisms, Brain Science Institute, RIKEN. *2017-2021 Post-doc, Lab. for systems neuropharmacology, Department of Pharmacology, Graduate school of medicine, Kyoto University. *2021-2023 Lecturer, Lab. for Cell regulation, Graduate school of Sciences, Nagoya Uni..."</p>
<p><b>New page</b></p><div>== Bibliography ==<br />
*2005 Graduated from Department of Biological sciences, Tokyo Metropolitan University<br />
*2010 Ph.D. Graduate School of Sciences, Tokyo Metropolitan University<br />
*2010-2017 Post-doc, Lab. for Memory Mechanisms, Brain Science Institute, RIKEN.<br />
*2017-2021 Post-doc, Lab. for systems neuropharmacology, Department of Pharmacology, Graduate school of medicine, Kyoto University.<br />
*2021-2023 Lecturer, Lab. for Cell regulation, Graduate school of Sciences, Nagoya University.<br />
*2023-present Specially Appointed Lecturer. Lab. for systems neuropharmacology, Department of Pharmacology, Graduate school of medicine, Kyoto University.<br />
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== Research topics ==<br />
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=== Molecular mechanisms for synaptic plasticity and Memory formation===<br />
Synaptic plasticity, such as synaptic potentiation and depression, are known as the fundamental mechanisms of memory formation, but their molecular regulatory mechanisms are not yet fully understood. In my research, I have focused on post-translational modifications of synaptic proteins, particularly phosphorylation, and have examined their spatiotemporal regulation. Of particular interest is the activation of CaMKII, also known as the major postsynaptic protein. It responds to the influx of calcium ions during learning, undergoes conformational changes, and exhibits kinase activity. As its downstream, phosphorylation of the AMPA-type glutamate receptor (AMPA receptor) has been highlighted, but its significance has remained unclear. Therefore, using the Phos-tag method, a quantitative analysis technique that I have employed in previous studies, I evaluated the level of phosphorylation at synapses. The results showed that the phosphorylation level is extremely low, suggesting limited involvement in memory formation.<br />
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=== Segregation of AMPA receptor and NMDA receptor as liquid-phase nanodomains===<br />
While AMPA receptors are known as the main mediators of information transmission, the NMDA-type glutamate receptor (NMDA receptor) is involved in plasticity. In my research, I have discovered a mechanism that the activation of CaMKII leads to the formation of distinct nanodomains for these receptors, allowing them to segregate by mutually excluding each other. Furthermore, these nanodomains possess properties of a liquid phase, ensuring fluidity, reactivity, and reversibility. This finding is consistent with the bidirectional nature of synaptic plasticity. Other constituent factors are also present within these nanodomains, and particularly, I have found that Neuroligin participated in the nanodomain of the AMPA receptor, which has the potential to concentrate signals on the AMPA receptor side while attenuating signals on the NMDA receptor side. This discovery provides a possible explanation for the simultaneous enhancement of synaptic strength and reduction of plasticity.<br />
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=== Postsynaptic liquid-phase nanodomains and their cross-talk ===<br />
Based on this background, I am driving research to elucidate the principles and significance of liquid-phase nanodomain formation and to explore strategies for manipulating them. Here are the research approaches I am pursuing:<br />
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1. Identification of nanodomain constituent factors using TurboID and comprehensive identification of binding interfaces using Alphafold-multimer.<br />
By employing TurboID, I aim to identify the factors involved in nanodomain assembly. Additionally, I utilize Alphafold-multimer to comprehensively identify the binding interfaces within these nanodomains among all PSD proteins.<br />
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2. Visualization of glutamate levels received by nanodomains using glutamate sensors.<br />
To understand the dynamics of nanodomain function, I employ glutamate biosensors to visualize the amount of glutamate received by these nanodomains.<br />
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3. Factors that disrupt nanodomains and their impact on synaptic strength.<br />
I am investigating the factors that can disrupt the segregation of nanodomains and examining their effects on synaptic strength. By understanding these significance, we can gain new insights into the regulation of synaptic plasticity.<br />
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4. Optical manipulation of nanodomains.<br />
I am exploring methods to optically control the formation and dispersion of nanodomains, enabling precise modulation of their properties and functions.<br />
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Through these research endeavors, I aim to deepen our understanding of liquid-phase nanodomain formation, elucidate their functional significance, and open the way for potential manipulations and applications in the context of synaptic plasticity.<br />
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== Publications ==<br />
# <pubmed>34975543</pubmed><br />
# <pubmed>33927400</pubmed><br />
# <pubmed>33752045</pubmed><br />
# <pubmed>33175445</pubmed><br />
# <pubmed>31879129</pubmed><br />
# <pubmed>30028513</pubmed><br />
# <pubmed>27641626</pubmed><br />
# <pubmed>27207106</pubmed><br />
# <pubmed>25533481</pubmed><br />
# <pubmed>24872417</pubmed><br />
# <pubmed>20200223</pubmed><br />
# <pubmed>20097924</pubmed><br />
# <pubmed>17868322</pubmed><br />
# <pubmed>17506859</pubmed><br />
# <pubmed>16802322</pubmed><br />
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== Funding & Awards ==<br />
Grant-in-Aid for start-up <br><br />
Grant-in-Aid for Young Scientists (B) <br><br />
Grant-in-Aid for international research promotion (A) <br><br />
Takeda foundation <br><br />
Narishige foundation <br><br />
Research-foundation for opto-science and technology <br><br />
Inamori foundation <br><br />
Kobayashi foundation <br><br />
<br><br />
<br><br />
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== Teaching Experience ==<br />
Class for basic biology <br><br />
Class for basic biochemistry <br><br />
Class for Journal reading <br><br />
Class for research presentation <br><br />
Experimental Class for heart <br><br />
Experimental Class for the observation of human blood <br><br />
Experimental Class for surgery and behavioral analysis of mouse <br><br />
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== Academic Society ==<br />
Society for Neuroscience (JP) <br><br />
Society for Neurochemistry (JP) <br><br />
Society for Biochemistry (JP) <br><br />
The molecular biology society Japan (JP) <br><br />
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== Personal Interests ==<br />
Muscle training, Playing with my 2yo-son<br />
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== Contact Address ==<br />
Department of Pharmacology<br><br />
Kyoto University Graduate School of Medicine<br><br />
Room 403, Building A<br><br />
Kyoto 606-8501 Japan<br />
<br />
E-mail: hosokawa.tomohisa.5s@kyoto-u.ac.jp</div>Yasunori Hayashi